sp. nov., isolated from soil of weathering dolomite in a karst area.

A Gram-stain-positive, aerobic actinomycete strain, designated KLBMP 8922, was isolated from a soil sample collected from weathering dolomite crust in Guizhou Province, PR China. KLBMP 8922 showed the 16S rRNA gene similarities to CCTCC AA 206006 (98.7 %), VN07A0015 (98.3 %) and M24DS4 (98.2 %). The taxonomic status of this strain was investigated by using a polyphasic approach. The aerial mycelia of KLBMP 8922 formed spore chains, and spores were cylindrical with smooth surfaces. The whole-cell sugars were ribose, mannose and galactose with traces of glucose and xylose. The diagnostic amino acids of the cell wall were ll-diaminopimelic acid, alanine and glutamic acid. The predominant menaquinones were MK-9(H) and MK-9(H). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. The major cellular fatty acids (>10 %) were iso-C, iso-C and iso-CH. The genomic DNA G+C content was 72.0 mol%. The digital DNA-DNA hybridization (dDDH) value between KLBMP 8922 and CCTCC AA 206006 was 24.1 %, and the average nucleotide identity (ANI) value was 81.0 %. On the basis of a combination of morphological, chemotaxonomic and phylogenetic characteristics, strain KLBMP 8922 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain was KLBMP 8922 (= CGMCC 1.19360 = NBRC 115572).