Volatile profile and micropropagation conditions of Link.

Unlabelled: Link. is a native South American plant, which possesses volatile compounds with pharmaceutical and medicinal properties such as antidiabetic and anti-inflammatory effects. However, the conservation and propagation of this plant are complicated by its recalcitrant seeds and delayed flowering transition. Hence, tissue culture is employed for the safe and efficient propagation of . However, the optimal conditions for the in vitro cultivation of remain unknown. Thus, this study aimed to characterize the volatile profile of adult field plants and evaluate the effects of different light intensities (43 and 70 μmol m s), gas exchange rates (14 and 25 µL L s), and exogenous sucrose concentrations (0, 20, and 30 g L) on their in vitro development. The results showed that β-caryophyllene is the major volatile compound produced by . Moreover, culturing in a medium containing 30 g L of sucrose and flasks with membranes that allow CO exchange at the rate of 25 µL L s produced vigorous and hardened plants with high survival rates independent of irradiance. This study is the first to report the optimal in vitro culture conditions for as a reference for future studies on micropropagation and secondary metabolite production using this species.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03634-8.