AI Article Synopsis
- Traditional light microscopy has limitations in accurately identifying spermatogonial phenotypes, which can create inconsistencies between molecular and physical observations.
- Improved identification could enhance the understanding of spermatogonial activity, bridging basic research with clinical applications.
- The chapter introduces two effective histological methods involving glutaraldehyde fixation and plastic resin embedding for better analysis of mammalian spermatogonial morphology and function.
Article Abstract
Numerous methods have been successfully used to evaluate mammalian spermatogonial biology However, the conventional light microscopy assays present a challenge in precisely identifying spermatogonial phenotypes, which can result in discrepancies between molecular and morphological findings. Such precise association could lead to a more robust interpretation of spermatogonial activity in steady-state spermatogenesis, which may facilitate the translation from basic research to clinical applications. In this chapter, we present two histological processing methods that enable a comprehensive analysis of spermatogonial morphology and function, involving fixation of mammalian testicular tissue in glutaraldehyde and embedding in plastic resin. These techniques have proven to be effective in light microscopy studies.
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| http://dx.doi.org/10.1007/978-1-0716-3139-3_2 | DOI Listing |
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