AI Article Synopsis
- The study examines the potential of human umbilical cord Wharton's Jelly-derived mesenchymal stem cells (HUMSCs) as a treatment for complications in extremely preterm infants, compared to term infants.
- Researchers isolated HUMSCs from both extremely preterm (22 to 28 weeks) and term infants (39 weeks) and analyzed their properties, such as proliferation rate and the expression of specific markers.
- Findings indicate that HUMSCs from extremely preterm infants have a higher proliferation rate and are more effective in promoting healing in damaged cells, suggesting their potential for use in cell therapy for preterm infants.
Article Abstract
Background: Despite the progress in perinatal-neonatal medicine, complications of extremely preterm infants continue to constitute the major adverse outcomes in neonatal intensive care unit. Human umbilical cord Wharton's Jelly-derived mesenchymal stem cells (HUMSCs) may offer new hope for the treatment of intractable neonatal disorders. This study will explore the functional differences of HUMSCs between extremely preterm and term infants.
Methods: UMSCs from 5 extremely preterm infants(weeks of gestation: 22 w,24 w,25 w,26 w,28 w) and 2 term infants(39 w,39 w) were isolated, and mesenchymal markers, pluripotent genes, proliferation rate were analyzed. HUVECs were injured by treated with LPS and repaired by co-cultured with HUMSCs of different gestational ages.
Results: All HUMSCs showed fibroblast-like adherence to plastic and positively expressed surface marker of CD105,CD73 and CD90, but did not expressed CD45,CD34,CD14,CD79a and HLA-DR; HUMSCs in extremely preterm exhibited significant increase in proliferation as evidenced by CCK8, pluripotency markers OCT-4 tested by RT-PCR also showed increase. Above all, in LPS induced co-cultured inflame systerm, HUMSCs in extremely preterm were more capable to promote wound healing and tube formation in HUVEC cultures, they promoted TGFβ1 expression and inhibited IL6 expression.
Conclusions: Our results suggest that HUMSCs from extremely preterm infants may be more suitable as candidates in cell therapy for the preterm infants.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352204 | PMC |
| http://dx.doi.org/10.1007/s13770-023-00538-9 | DOI Listing |
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