AI Article Synopsis
- Let-7a is a microRNA that's less expressed in cancer cells and shows promise as a biomarker and treatment target for tumor suppression.
- The study developed detection methods for let-7a using a hybridization chain reaction (HCR) combined with techniques like lateral flow assay (LFA) and electrochemical impedance spectroscopy.
- The results indicated that the detection methods could identify let-7a concentrations ranging from 10.0 fM to 1.0 nM, with a sensitivity limit of 4.2 fM.
Article Abstract
Let-7a, a type of low-expressed microRNAs in cancer cells, has been investigated as a promising biomarker and therapeutic target for tumor suppression. Developing simple and sensitive detection methods for let-7a is important for cancer diagnosis and treatment. In this work, the hybridization chain reaction (HCR) was initiated by let-7a via two hairpin primers (H1 and H2). After the HCR, the remaining hairpin H1 was further detected by lateral flow assay (LFA) and electrochemical impedance spectroscopy. For LFA, biotin-modified H1(bio-H1) and free H2 were used for HCR. With the decrease of let-7a concentration, the color of T line gradually increased. As for electrochemical methods, the H1'-AuNP-modified electrode was used for detection of bio-H1 based on the difference of impedance (Δ) detected without and with different concentrations of let-7a participating in the HCR. This method could detect let-7a in the range of 10.0 fM and 1.0 nM with detection limits of 4.2 fM.
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| http://dx.doi.org/10.1021/acs.jmedchem.3c00499 | DOI Listing |
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