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Development of a novel modified selective medium cefixime-tellurite-phosphate-xylose-rhamnose MacConkey agar for isolation of Escherichia albertii and its evaluation with food samples.
Int J Food Microbiol
January 2025
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58, Rinkuourai-kita, Izumisano, Osaka 598-8531, Japan; Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan; Asian Health Science Research Institute, Osaka Metropolitan University, Osaka, Japan; Osaka International Research Center for Infectious Diseases, Osaka Metropolitan University, 1-58, Rinkuourai-kita, Izumisano, Osaka 598-8531, Japan. Electronic address:
Since cefixime and tellurite are known to inhibit most bacteria belonging to Enterobacterales, we found that addition of tellurite inhibited E. albertii growth in Luria Bertani broth but not in tryptic soy broth (TSB), and addition of phosphate and soy peptone enhanced E. albertii growth in TSB in presence of tellurite.
View Article and Find Full Text PDFEfficient calcium fumarate overproduction from xylose and corncob-derived xylose by engineered strains of Aureobasidium pullulans var. Aubasidani DH177.
Microb Cell Fact
December 2024
College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
Article Synopsis
- Xylose, derived from lignocellulose, is a crucial renewable resource for producing valuable bioproducts like fumaric acid; optimizing its conversion is essential.
- The study identified a genetically modified strain (TKL-4) of A. pullulans that effectively uses xylose and corncob-derived xylose to produce calcium fumarate, demonstrating higher yields compared to glucose.
- The TKL-4 strain achieved impressive fermentation results, generating up to 88.5 g/L of calcium fumarate from xylose during a 10-liter fermentation process, showcasing its potential for eco-friendly bioproduct development.
Co-substrate model development and validation on pure sugars and corncob hemicellulosic hydrolysate for xylitol production.
Sci Rep
October 2024
Center of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand.
A co-substrate model of Candida tropicalis TISTR 5306 cultivated in 10 - 100 g/L xylose and 1 - 10 g/L glucose at the ratio of 10:1 was developed based in part on modified Monod equation. The kinetic parameters include substrate limitation as well as substrate and product inhibitions with inclusion of threshold values. A general good fitting with average RSS, R, and MS values of 162, 0.
View Article and Find Full Text PDFIdentification of a novel NADPH generation reaction in the pentose phosphate pathway in using mBFP.
J Bacteriol
November 2024
Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Suita, Osaka, Japan.
NADPH is a redox cofactor that drives the anabolic reactions. Although major NADPH generation reactions have been identified in , some minor reactions have not been identified. In the present study, we explored novel NADPH generation reactions by monitoring the fluorescence dynamics after the addition of carbon sources to starved cells, using a metagenome-derived blue fluorescent protein (mBFP) as an intracellular NADPH reporter.
View Article and Find Full Text PDFPseudoalteromonas simplex sp. nov. Isolated from the Skin of Bandtail Puffer Fish (Sphoeroides spengleri).
Curr Microbiol
October 2024
Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Article Synopsis
- - A new bacterial isolate named A520 was discovered from the skin of the bandtail puffer fish in Brazil, characterized as Gram-negative, flagellated, and aerobic, with optimal growth conditions at 25-30 °C and 3% salt.
- - The genome of A520 has a size of 4.5 Mb, containing 4082 coding genes, and shows high similarity (97.9%) to the bacteria Pseudoalteromonas shioyasakiensis, indicating it may belong to a related lineage.
- - This novel species, named Pseudoalteromonas simplex sp. nov., exhibits unique phenotypic features and contains gene clusters for bioactive compounds that may relate to the ecological interactions
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