A variety of in vivo and in vitro methods: radioactive iodine accumulation, thyroid suppression and stimulation test, a scintigraphic study and a radioimmunoassay to determine triiodothyronine-binding capacity (free T3), thyroxine (T4) concentration and the effective thyroxine ratio (ETR), was used for diagnostic specification and accurate planning of therapy of patients with thyroid diseases. The suppression test combined with the results of the in vitro analysis (free T3, T4 and ETR) was decisive for establishing diagnosis.

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