AI Article Synopsis
- Canker, caused by the fungus (Feltgen) J.K. Stone, is a major threat to blueberry crops and was studied for its characteristics and genetic relationships.
- Infected blueberry stems were gathered from various regions in Poland between 2016 and 2020, leading to the identification and testing of 24 fungal isolates based on morphology and molecular techniques, including PCR.
- The study found that conidia (spores) had an average size of 9.36 x 2.45 µm, with various shapes, and the fungus exhibited the fastest growth on specific media (SNA and PCA), while molecular analysis confirmed a 100% similarity to a known reference strain in GenBank.
Article Abstract
canker caused by (Feltgen) J.K. Stone is considered one of the most dangerous diseases of blueberry crops. The purpose of the study was the phenotypic characterization and phylogenetic analysis of this fungus. Infected stems were collected from blueberry crops in the Mazovian, Lublin, and West Pomeranian Voivodships in 2016-2020. Twenty-four isolates were identified and tested. The isolates were identified on the basis of their morphology and molecular characteristics (PCR). The average conidia size was 9.36 ± 0.81 × 2.45 ± 0.37 µm. The conidia were hyaline, ellipsoid or straight, two-celled, rounded, or terminally pointed. The pathogen growth dynamics were tested on six media: PDA, CMA, MEA, SNA, PCA, and Czapek. The fastest daily growth of fungal isolates was observed on SNA and PCA, and the slowest on CMA and MEA. Pathogen rDNA amplification was performed with ITS1F and ITS4A primers. The obtained DNA sequence of the fungus showed 100% nucleotide similarity to the reference sequence deposited in the GenBank. Molecular characterization of isolates was performed for the first time in this study.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10222062 | PMC |
| http://dx.doi.org/10.3390/pathogens12050642 | DOI Listing |
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