Calcium (Ca) flux acts as a central signaling pathway in B cells, and its alterations are associated with autoimmune dysregulation and B-cell malignancies. We standardized a flow-cytometry-based method using various stimuli to investigate the Ca flux characteristics of circulating human B lymphocytes from healthy individuals. We found that different activating agents trigger distinct Ca flux responses and that B-cell subsets show specific developmental-stage dependent Ca flux response patterns. Naive B cells responded with a more substantial Ca flux to B cell receptor (BCR) stimulation than memory B cells. Non-switched memory cells responded to anti-IgD stimulation with a naive-like Ca flux pattern, whereas their anti-IgM response was memory-like. Peripheral antibody-secreting cells retained their IgG responsivity but showed reduced Ca responses upon activation, indicating their loss of dependence on Ca signaling. Ca flux is a relevant functional test for B cells, and its alterations could provide insight into pathological B-cell activation development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219102 | PMC |
| http://dx.doi.org/10.3390/ijms24109107 | DOI Listing |
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