Synthesis, Antimicrobial, and Antibiofilm Activities of Some Novel 7-Methoxyquinoline Derivatives Bearing Sulfonamide Moiety against Urinary Tract Infection-Causing Pathogenic Microbes.

A new series of 4-((7-methoxyquinolin-4-yl) amino)--(substituted) benzenesulfonamide was synthesized via the reaction of 4-chloro-7-methoxyquinoline with various sulfa drugs. The structural elucidation was verified based on spectroscopic data analysis. All the target compounds were screened for their antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and unicellular fungi. The results revealed that compound has the highest effect on most tested bacterial and unicellular fungal strains. The highest effect of compound was observed against and with MIC = 7.812 and 31.125 µg/mL, respectively. Compounds and showed broad-spectrum antimicrobial activity, but the activity was lower than that of . The antibiofilm activity of compound was measured against different pathogenic microbes isolated from the urinary tract. Compound could achieve biofilm extension at its adhesion strength. After adding 10.0 µg/mL of compound , the highest percentage was 94.60% for 91.74% for and 98.03% for . Moreover, in the protein leakage assay, the quantity of cellular protein discharged from was 180.25 µg/mL after treatment with 1.0 mg/mL of compound , which explains the creation of holes in the cell membrane of and proves compound 's antibacterial and antibiofilm properties. Additionally, in silico ADME prediction analyses of compounds , and revealed promising results, indicating the presence of drug-like properties.