AI Article Synopsis
- * The study examined how melatonin protects sheep granulosa cells from oxidative damage and apoptosis (cell death), revealing that high concentrations of HO induce apoptosis, which melatonin can counteract.
- * Through advanced sequencing, 109 genes were identified as significantly influenced by melatonin's protective effects, with specific genes linked in a regulatory relationship impacting its action against apoptosis via the MAP3K8-FOS pathway.
Article Abstract
Melatonin is not only a highly effective active oxygen scavenger but also an important reproductive hormone. Melatonin has a regulatory effect on animal reproduction, especially on the ovaries. It can affect the proliferation and apoptosis of cells in follicles. However, the mechanisms of the dual antioxidation and anti-apoptosis effects of melatonin on granulosa cells are still not clear, especially in sheep. Therefore, we investigated the mechanisms of the protective effect of melatonin against oxidative damage in granulosa cells. At a concentration of 250 µmol/L, HO promoted granulosa cell apoptosis; however, 10 ng/mL melatonin effectively alleviated the pro-apoptotic effect of HO. Furthermore, through the application of high-throughput sequencing technology, we identified 109 significantly differentially expressed genes (35 upregulated and 74 downregulated genes) involved in the protective effect of melatonin against apoptosis. The expression levels of nine related genes, i.e., , , , , , , , , and , changed significantly. and gene overexpression impacted the protective effect of melatonin in granulosa cells; the two genes exhibited an upstream and downstream regulatory relationship. Our findings indicated that melatonin alleviated HO-induced apoptosis in sheep granulosa cells through the MAP3K8-FOS pathway.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218642 | PMC |
| http://dx.doi.org/10.3390/genes14051067 | DOI Listing |
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