Antimicrobial resistance is a major public health concern. The aim of this study was to assess the presence of antibiotic resistance genes, previously reported in in gastric samples of 36 pigs, in which DNA of -like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the mutation gene, conferring tetracycline resistance, and one sample was positive for the gene with a single nucleotide polymorphism, conferring metronidazole resistance. All three amplicons showed the highest homology with -associated antibiotic resistance gene sequences. These findings indicate that acquired antimicrobial resistance may occur in -like organisms associated with pigs.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215371PMC
http://dx.doi.org/10.3390/antibiotics12050906DOI Listing

Publication Analysis

Top Keywords

tetracycline resistance
8
resistance genes
8
gastric samples
8
samples pigs
8
antimicrobial resistance
8
antibiotic resistance
8
-like organisms
8
resistance
7
molecular detection
4
detection metronidazole
4

Similar Publications

spp. are facultative pathogens that contribute to the pathogenesis of multiple bovine diseases, including the bovine respiratory disease complex, and have been shown to form biofilms. Biofilm formation is associated with increased antibiotic resistance in many organisms, but accurate determination of antimicrobial susceptibility in biofilms is challenging.

View Article and Find Full Text PDF

Antibiotic susceptibilities, hemolytic activities, and technological properties of 46 isolates from kimchi were evaluated to select starter candidates. All strains were susceptible to clindamycin and erythromycin, while some exhibited resistance to ampicillin, chloramphenicol, gentamicin, streptomycin, and tetracycline; all were resistant to kanamycin based on the EFSA breakpoint values for species. PCR analysis did not detect resistance genes for these six antibiotics in any strain.

View Article and Find Full Text PDF

, a significant cause of gastroenteritis and a growing public health concern, has become increasingly prevalent owing to the rise in ocean temperatures driven by climate change. This study aimed to characterize the genetic diversity, pathogenic potential, and antimicrobial resistance (AMR) profiles of strains isolated from the southern coastal region of Korea. Using whole genome sequencing (WGS) and advanced bioinformatics tools, we identified novel sequence types through multilocus sequence typing and serotyped isolates using the VPsero database.

View Article and Find Full Text PDF

Multilocus Sequence Typing and Antimicrobial Susceptibility of Isolated from Foods Surveyed in Kosovo.

Microorganisms

November 2024

Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.

In the absence of data on the reporting of resistance to antibiotics, we sought to determine which clonal complexes (CCs)/sequence types (STs) circulate in the food chain in Kosovo and to determine their antibiogram profiles to a panel of 18 antibiotics. From a total of 114 isolates, 21 different typical STs were identified by multilocus sequence typing (MLST). Each isolate derived from the food categories was subjected to tests to verify its susceptibility to the selected antibiotics according to the designed Sensititre GPN3F panel.

View Article and Find Full Text PDF

Antimicrobial resistance (AMR) has become precarious, warranting investments in antimicrobial discovery. To investigate the antibacterial activity of rosemary essential oil (REO), alone and in combination with selected conventional antibiotics. REO was subjected to antimicrobial susceptibility testing (including minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) determination) and investigation of anti-pre-biofilm and antibiofilm activities.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!