Few studies have addressed drug resistance of Enterobacterales in rural communities in developing countries. This study aimed to determine the coexistence of extended-spectrum β-lactamase (ESBL) and carbapenemase genes in and strains carrying the gene in rural communities in Ecuador from healthy humans and their backyard animals. Sixty-two strains, thirty and thirty-two strains carrying the gene were selected from a previous study. PCR were performed for the presence of ESBLs and carbapenemase genes. The strains were further characterized, and the genetic relationship was studied with multi-locus sequencing typing (MLST) of seven housekeeping genes. Fifty-nine of the sixty-two isolates (95%) harbored at least on β-lactam resistance gene. The most prevalent ESBL genes were the genes (present in in 80% of the strains) and the gene (present in 84% of the strains). MSLT analysis revealed 28 different sequence types (ST); 15 for and 12 for , with most ST never described in humans and animals. The coexistence of and β-lactams resistant genes in and strains is alarming and threatens the efficacy of last-resort antibiotics. Our findings highlight backyard animals as a reservoir of β-lactams resistant genes.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215259PMC
http://dx.doi.org/10.3390/antibiotics12050856DOI Listing

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