AI Article Synopsis

  • Tumor-on-chips are valuable for cancer research but face challenges in fabrication and usability.
  • A new 3D-printed chip allows for better mixing and realistic concentration profiles, supporting tissue growth.
  • Tests showed that this chip can effectively host microtumors and evaluate drug responses, highlighting its potential for studying cancer biology.

Article Abstract

Tumor-on-chips have become an effective resource in cancer research. However, their widespread use remains limited due to issues related to their practicality in fabrication and use. To address some of these limitations, we introduce a 3D-printed chip, which is large enough to host ~1 cm of tissue and fosters well-mixed conditions in the liquid niche, while still enabling the formation of the concentration profiles that occur in real tissues due to diffusive transport. We compared the mass transport performance in its rhomboidal culture chamber when empty, when filled with GelMA/alginate hydrogel microbeads, or when occupied with a monolithic piece of hydrogel with a central channel, allowing communication between the inlet and outlet. We show that our chip filled with hydrogel microspheres in the culture chamber promotes adequate mixing and enhanced distribution of culture media. In proof-of-concept pharmacological assays, we biofabricated hydrogel microspheres containing embedded Caco2 cells, which developed into microtumors. Microtumors cultured in the device developed throughout the 10-day culture showing >75% of viability. Microtumors subjected to 5-fluorouracil treatment displayed <20% cell survival and lower VEGF-A and E-cadherin expression than untreated controls. Overall, our tumor-on-chip device proved suitable for studying cancer biology and performing drug response assays.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215397PMC
http://dx.doi.org/10.3390/bioengineering10050554DOI Listing

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