Characterization of polymorphisms in the follicle-stimulating hormone receptor and insulin-like growth factor-1 genes and their association with fertility traits in Jawa-Brebes cows.

Background And Aim: The availability of fertility markers is crucial for maintaining, protecting, and improving the genetics of Jawa-Brebes (Jabres) cows. Follicle-stimulating hormone receptor () and insulin-like growth factor-1 () play critical roles in female reproductive physiology. The single-nucleotide polymorphisms (SNPs) and correlate with cows' fertility traits. This study aimed to identify these SNPs and their potential associations with fertility parameters in Jabres cows.

Materials And Methods: Samples were collected from 45 heads of multiparous Jabres cows aged 3-10 years with body condition scores of 2.5-5.0 on a 5-point scale in Brebes Regency, Java, Indonesia. These cows were assigned to fertile (n = 16) and infertile groups (n = 29). Polymerase chain reaction (PCR) was carried out for DNA amplification of and fragments. Restriction fragment length polymorphism-PCR with the restriction enzymes for the product of and for the product of was used to identify SNPs.

Results: The enzyme cut the 211 bp DNA fragment of in all samples into two bands of 128 bp and 83 bp (GG genotype). Meanwhile, the genotyping of amplicon products of generated a single 249 bp fragment (CC genotype) in both groups.

Conclusion: The results showed that the and loci were monomorphic in Jabres cows. Thus, neither nor is a possible genetic marker for fertility in Jabres cows.