Several important properties of the common pili from Escherichia coli are discussed. These pili were resistant to the gentle Folin-Ciocalteau reagent methods for protein detection and were not readily solubilized by sodium dodecyl sulfate. They were found to contain a reducing sugar but not peptidoglycan. The pilin had multiple conformations in sodium dodecyl sulfate solution, and the appearance of multiple bands on sodium dodecyl sulfate gels did not necessarily indicate heterogeneity of the preparation. The ilus subunit was found to be a different protein than outer membrane III, which has the same apparent molecular weight. In addition, we conformed the results of Brinton (Trans. N.Y. Acad. Sci 27:1003-1054, 1965): that there is a dramatic change in the properties of pili after they are heated at pH values below 2.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC218129 | PMC |
| http://dx.doi.org/10.1128/jb.138.3.969-975.1979 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Preparation and characterization of a rat uterine decellularized scaffold.
Gland Surg
December 2024
Department of Gynecology, Wenzhou People's Hospital, Wenzhou, China.
Background: Infertility is a special reproductive health defect. For women, congenital uterine malformations, extensive adhesions in the uterine cavity, and hysterectomy are associated with infertility. Uterine transplantation is technically feasible, but its clinical application and development are limited by donor shortages and immune rejection.
View Article and Find Full Text PDFSustainability assessment of the micellar optimized HPLC method for concomitant quantification of a common triple regimen for cardiovascular disorders: application to marketed formulations and dissolution testing.
Anal Methods
January 2025
Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
Hypertension and dyslipidemia are two of the most frequently co-occurring cardiovascular risk factors. The combined regimen of hydrochlorothiazide (HCTZ), rosuvastatin (ROS), and losartan (LOS) helped in the successful management of both conditions. This work's objective is to develop an eco-friendly, sensitive, simple, and reliable chromatographic method for the simultaneous estimation of HCTZ, ROS, and the LOS ternary mixture in their pure form, and pharmaceutical formulations.
View Article and Find Full Text PDFEnhancing Droplet Spreading on a Hydrophobic Plant Surface by Surfactant/Cellulose Nanocrystal Complexes.
ACS Nano
January 2025
Department of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
A surfactant is an efficient and common additive used to enhance the spreading of droplets on hydrophobic surfaces. However, a high surfactant concentration is required to achieve the desired performance, resulting in environmental pollution and increased costs. Additionally, the pesticide loading capacity of surfactants at low concentrations (below their critical micelle concentrations) is a concern.
View Article and Find Full Text PDFA Comparative Review on the Production of Factor VIII in Human and Non-human Hosts.
Curr Pharm Des
January 2025
School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
Hemophilia A (HA) is an inherited condition that is characterized by a lack of coagulation factor VIII (FVIII), which is needed for blood clotting. To produce recombinant factor VIII (rFVIII) for treatment, innovative methods are required. This study presents a thorough examination of the genetic engineering and biotechnological methods that are essential for the production of this complex process.
View Article and Find Full Text PDFInvestigation and elimination of noncovalent artificial aggregates during non-reduced capillary electrophoresis-sodium dodecyl sulfate analysis of a multi-specific antibody.
J Pharm Biomed Anal
January 2025
State Key Laboratory of Neurology and Oncology Drug Development, Nanjing, China; Simcere Zaiming Pharmaceutical Co, Ltd., Nanjing, China. Electronic address:
Capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) is widely used in the biopharmaceutical industry for monitoring purity and analyzing impurities. The accuracy of the method may be compromised by artificial species resulting from sample preparation or electrophoresis separation due to suboptimal conditions. During non-reduced CE-SDS analysis of a multispecific antibody (msAb), named as multispecific antibody C (msAb-C), a cluster of unexpected peaks was observed after the main peak.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!