Rapeseed is one of the most important oil crops in the world. Increasing demand for oil and limited agronomic capabilities of present-day rapeseed result in the need for rapid development of new, superior cultivars. Double haploid (DH) technology is a fast and convenient approach in plant breeding as well as genetic research. is considered a model species for DH production based on microspore embryogenesis; however, the molecular mechanisms underlying microspore reprogramming are still vague. It is known that morphological changes are accompanied by gene and protein expression patterns, alongside carbohydrate and lipid metabolism. Novel, more efficient methods for DH rapeseed production have been reported. This review covers new findings and advances in DH production as well as the latest reports related to agronomically important traits in molecular studies employing the double haploid rapeseed lines.
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http://dx.doi.org/10.3390/cimb45050282 | DOI Listing |
Sci Rep
December 2024
College of Agronomy, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China.
Brace roots are the primary organs for water and nutrient absorption, and play an important role in lodging resistance. Dissecting the genetic basis of brace root traits will facilitate breeding new varieties with lodging resistance and high yield. In present study, genome-wide association study (GWAS) and genomic selection (GS) for brace root penetrometer resistance (PR), root number (RN), and tier number (TN) were conducted in a multi-parent doubled haploid (DH) population.
View Article and Find Full Text PDFBiol Methods Protoc
December 2024
Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC), Selektsionnaya St, 14, VNIISSOK, Odintsovo Reg., 143072 Moscow, Russia.
In this protocol for obtaining doubled haploids plants (DH), we propose a new method for microspore isolation. This method is useful for genotypes of the Brassicaceae family with low responsiveness to DH technology. For such crops, it allows increasing the embryo yield several times and sometimes obtaining embryos for the first time.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources (VIR), 190000 Saint Petersburg, Russia.
In barley having adherent hulls, an irreversible connection between the pericarp with both palea and lemma is formed during grain maturation. A mutation in the () gene prevents this connection and leads to the formation of barley with non-adherent hulls. A genetic model of two isogenic lines was used to elucidate the genetic mechanisms of hull adhesion: a doubled haploid line having adherent hulls and its derivative with non-adherent hulls obtained by targeted mutagenesis of the gene.
View Article and Find Full Text PDFTheor Appl Genet
December 2024
Department of Agronomy, Iowa State University, Ames, IA, 50011, USA.
Restoration of haploid female and haploid male fertility without colchicine is feasible. Three SNPs and eight gene models for HFF, and one SNP and a gene model for HMF were identified. Doubled haploid (DH) breeding accelerates the development of elite inbred lines and facilitates the incorporation of exotic germplasm, offering a powerful tool for maize improvement.
View Article and Find Full Text PDFGene
February 2025
College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China. Electronic address:
Maize is one of the most important crops for human food, animal feed, and industrial raw materials. Ear shank length (ESL) and ear shank node number (ESNN) are crucial selection criteria in maize breeding, impacting grain yield and dehydration rate during mechanical harvesting. To unravel the genetic basis of ESL and ESNN in maize, an association panel consisting of 379 multi-parent doubled-haploid (DH) lines was developed for genome-wide association studies (GWAS) and genomic selection (GS).
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