AI Article Synopsis
- N-myristoyltransferases (NMTs) are enzymes that convert myristoyl-CoA into myristoylated proteins, playing a critical role in guiding proteins to cell membranes.
- NMTs are part of the GCN5-related N-acetyltransferase family and are known for their ability to also interact with unusual substrates like lysine and acetyl-CoA.
- The chapter discusses the kinetic methods used to study and understand the specific catalytic activities of NMTs in laboratory settings.
Article Abstract
N-myristoyltransferases (NMTs) are members of the large family of GCN5-related N-acetyltransferases (GNATs). NMTs mainly catalyze eukaryotic protein myristoylation, an essential modification tagging protein N-termini and allowing successive subcellular membrane targeting. NMTs use myristoyl-CoA (C14:0) as major acyl donor. NMTs were recently found to react with unexpected substrates including lysine side-chains and acetyl-CoA. This chapter details the kinetic approaches that have allowed the characterization of the unique catalytic features of NMTs in vitro.
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| http://dx.doi.org/10.1016/bs.mie.2023.02.018 | DOI Listing |
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