https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=37213683&retmode=xml&tool=Litmetric&email=readroberts32@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09 3721368320230523
2662-68101052023MayHorticulture researchHortic ResThe first chromosome-level Fallopia multiflora genome assembly provides insights into stilbene biosynthesis.uhad047uhad047uhad04710.1093/hr/uhad047Fallopia multiflora (Thunb.) Harald, a vine belonging to the Polygonaceae family, is used in traditional medicine. The stilbenes contained in it have significant pharmacological activities in anti-oxidation and anti-aging. This study describes the assembly of the F. multiflora genome and presents its chromosome-level genome sequence containing 1.46 gigabases of data (with a contig N50 of 1.97 megabases), 1.44 gigabases of which was assigned to 11 pseudochromosomes. Comparative genomics confirmed that F. multiflora shared a whole-genome duplication event with Tartary buckwheat and then underwent different transposon evolution after separation. Combining genomics, transcriptomics, and metabolomics data to map a network of associated genes and metabolites, we identified two FmRS genes responsible for the catalysis of one molecule of p-coumaroyl-CoA and three molecules of malonyl-CoA to resveratrol in F. multiflora. These findings not only serve as the basis for revealing the stilbene biosynthetic pathway but will also contribute to the development of tools for increasing the production of bioactive stilbenes through molecular breeding in plants or metabolic engineering in microbes. Moreover, the reference genome of F. multiflora is a useful addition to the genomes of the Polygonaceae family.© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University.ZhaoYujiaoYSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei 230012, China.YangZhengyangZSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.ZhangZhongrenZNovogene Bioinformatics Institute, Beijing 301700, China.YinMinzhenMSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.ChuShanshanSSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei 230012, China.TongZhenzhenZSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.QinYuejianYSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.ZhaLiangpingLSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei 230012, China.FangQingyingQSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei 230012, China.YuanYuanYNational Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.HuangLuqiLNational Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.PengHuashengHSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.Research Unit of DAO-DI Herbs, Chinese Academy of Medical Sciences, 2019RU57, Beijing 100700, China.engfigshare10.6084/m9.figshare.19720381Journal Article20230315
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