AI Article Synopsis
- Gold nanoparticles were enhanced using the amino acid tryptophan and vitamin riboflavin, resulting in a 65% increase in resonance energy transfer (RET) efficiency.
- The presence of these nanoparticles altered the photobleaching dynamics of fluorescent molecules, making them detectable in biological materials with autofluorescent compounds.
- Using advanced deep-ultraviolet fluorescence microscopy, researchers could classify fluorescent centers in cancer cells to identify areas where the nanoparticles accumulated, despite their small size.
Article Abstract
Gold nanoparticles were functionalized by amino acid tryptophan and vitamin riboflavin - a resonance energy transfer (RET) pair of biomolecules. The presence of the gold nanoparticles resulted in 65% increase in RET efficiency. Because of enhanced RET efficiency, the photobleaching dynamics of the fluorescent molecules at the surface of the nanoparticles is different from that of molecules in solution. The observed effect was used for detection of the functionalized nanoparticles within biological material rich with autofluorescent species. Synchrotron radiation deep-ultraviolet fluorescence microscopy is used to study the photobleaching dynamics of the fluorescence centers within human hepatocellular carcinoma Huh7.5.1 cells incubated with the nanoparticles. The fluorescent centers were classified according to their photobleaching dynamics, which enabled the discrimination of the cell areas where the accumulation of the nanoparticles takes place, even though the particles were smaller than the spatial resolution of the images.
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| http://dx.doi.org/10.1016/j.colsurfb.2023.113340 | DOI Listing |
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