Selecting proper genome assembly is key for downstream analysis in genomics studies. However, the availability of many genome assembly tools and the huge variety of their running parameters challenge this task. The existing online evaluation tools are limited to specific taxa or provide just a one-sided view on the assembly quality. We present WebQUAST, a web server for multifaceted quality assessment and comparison of genome assemblies based on the state-of-the-art QUAST tool. The server is freely available at https://www.ccb.uni-saarland.de/quast/. WebQUAST can handle an unlimited number of genome assemblies and evaluate them against a user-provided or pre-loaded reference genome or in a completely reference-free fashion. We demonstrate key WebQUAST features in three common evaluation scenarios: assembly of an unknown species, a model organism, and a close variant of it.
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http://dx.doi.org/10.1093/nar/gkad406 | DOI Listing |
J Immunother Cancer
March 2025
Laboratory of Targeted Therapy and Precision Medicine, Department of Clinical Laboratory, Shanghai 6th Peoples Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China
Background: The emergence of immunotherapy has revolutionized the paradigm of cancer treatment with immune checkpoint blockades (ICB) in solid cancers, including colorectal cancer (CRC). However, only a small subset of CRC patients harboring deficient mismatch repair (dMMR) or microsatellite instability-high (MSI-H) benefits from ICB therapy. A very limited response to ICB therapy has been achieved in MMR-proficient CRC, representing a significant challenge limiting the clinical application of immunotherapy.
View Article and Find Full Text PDFBMJ Glob Health
March 2025
Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania, United Republic of.
Background: Addressing sickle cell disease (SCD) is crucial for achieving health-related Sustainable Development Goals, particularly in Africa. The region is significantly affected, with 78.7% of patients with SCD residing in sub-Saharan Africa and over 515 000 newborns diagnosed annually.
View Article and Find Full Text PDFBiochem Biophys Res Commun
March 2025
Nanhu Laboratory, National Center of Biomedical Analysis, Beijing, 100850, China. Electronic address:
The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global public health crisis. The nucleocapsid (N) protein plays a pivotal role in a variety of biological processes in the life cycle of SARS-CoV-2, such as viral assembly. In this study, we investigated the liquid-liquid phase separation (LLPS) capacity of the N protein of seven SARS-CoV-2 strains, including the variants of concern (VOC) and interest (VOI), and its impact on viral replication.
View Article and Find Full Text PDFWellcome Open Res
February 2025
Natural History Museum, London, England, UK.
We present a genome assembly from a male (Common Sheetweb Spider Arthropoda; Arachnida; Araneae; Linyphiidae). The genome sequence has a total length of 1,349.10 megabases.
View Article and Find Full Text PDFWellcome Open Res
January 2025
Royal Botanic Gardens Kew, Richmond, England, UK.
We present a genome assembly from a specimen of (Greek sea-spurrey; Streptophyta; Magnoliopsida; Caryophyllales; Caryophyllaceae). The genome sequence has a total length of 466.20 megabases.
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