Isolation and identification of microbiota of for their application as paratransgenic tools in vector control.

Background And Objectives: Although the study on the bacteria residing in the mid-gut, salivary gland, and reproductive organs of insect vectors have drawn appeal to the host-pathogen interactions, we know comparatively less about microbiota that naturally exist in different mosquito organs within Iran.

Materials And Methods: In the current investigation, PCR assay by using 16S rRNA gene amplification and DNA sequencing, in addition to the traditional culture-based approach utilized for the detection of cultivable bacterial assemblages in mid-gut and reproductive tracts of

Results: The identified bacteria isolated from different tissues of 45 individuals were consisted of and The results showed that Proteobacteria was the most prevalent phylum in both genders' mid-gut and reproductive tracts, and was the most common bacteria that originated in adult females and males' tissues.

Conclusion: These outcomes recommend that the discovered microbiome may span through populations. This data can be utilized to interfere with the transmission of pathogens and design new strategies for the control of mosquito-borne diseases.