Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Three bacterial strains, H21R-40 and H21R-36 from garlic () and H25R-14 from onion (), were isolated from plant rhizospheres sampled in the Republic of Korea. Results of 16S rRNA gene sequence analysis revealed the highest sequence similarity of strain H21R-40 to subsp. CBX151 (97.3 %) and JW-1 (97.2 %), and strain H25R-14 to HDW9B (98.8 %) and Re6 (98.4 %), while the sequence similarity between strains H21R-40 and H21R-36 was 99.8 %. According to the phylogenomic tree, strains H21R-40 with H21R-36 formed an independent clade separable from other species within the genus and strain H25R-14 clustered with HDW9B, HDW9A and HDW9C. Strains H21R-40 and H21R-36 had orthologous average nucleotide identity (OrthoANI) and digital DNA-DNA hybridization (dDDH) values (98.1 % and 86.9 %, respectively) higher than the threshold ranges for species delineation (95-96 % and 70 %, respectively). The OrthoANI and dDDH values between two strains (H21R-40 and H25R-14) and the type strains of species of the genus were lower than 81 and 24 %, respectively. The peptidoglycan type of three strains was type B1. The major menaquinones and major polar lipids of the strains were MK-11 and MK-10, and diphosphatidylglycerol, phatidylglycerol and an unidentified glycolipid, respectively. The major fatty acids (more than 10 % of the total fatty acids) of strains H21R-40 and H21R-36 were anteiso-C, anteiso-C and iso-C, and those of strain H25R-14 were anteiso-C and iso-C. The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that the strains represent two novel species of the genus , named sp. nov. (H21R-40 and H21R-36) and sp. nov. (H25R-14). The respective type strains are H21R-40 (=DSM 114348=JCM 35241=KACC 21839=NBRC 115481) and H25R-14 (=DSM 114346=JCM 35239=KACC 21837=NBRC 115479).
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Source |
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http://dx.doi.org/10.1099/ijsem.0.005883 | DOI Listing |
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