AI Article Synopsis
- The text presents a novel method for colorimetric detection of bisphenol A (BPA) using a combination of catalytic hairpin assembly (CHA) and a hybridization chain reaction (HCR) involving DNAzymes.
- The method exploits the binding of BPA to a specific aptamer hairpin, which initiates the formation of Y-shaped DNA structures that can generate multiple fragments and lead to the assembly of longer DNA strands.
- Optimized detection conditions allow this biosensor to achieve high sensitivity in detecting BPA at concentrations as low as 0.2 pM, indicating its potential for ultrasensitive applications.
Article Abstract
An efficient and innovative strategy for colorimetric detection of bisphenol A (BPA) is shown here based on target-induced catalytic hairpin assembly (CHA) and DNAzyme-caused fragment self-assembly hybridization chain reaction (HCR). BPA can bind with its aptamer hairpin to trigger CHA, thus forming Y-shaped DNA nanostructures with an enzyme-strand (E-DNA) tail. Subsequently, the E-DNA can cyclically cleave the substrate hairpin, generating many fragments which can cause self-assembly HCR to form long strand DNA. Finally, the formed long strand DNA can hybridize with short single strand DNA on AuNPs, causing the color change of AuNPs from red to blue. Six important detection conditions of the proposed aptasensor were optimized. Under optimal conditions, the biosensor has high sensitivity for BPA detection at concentrations ranging from 0.8 pM to 500 pM and the detection limit is as low as 0.2 pM, providing a promising prospective ultrasensitive detection of BPA.
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| http://dx.doi.org/10.1039/d3ay00409k | DOI Listing |
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