Assessing the effectiveness of green synthesized zinc oxide nanoparticles in controlling multidrug-resistant clinical bacteria.

Unlabelled: Multi-drug resistant pathogenic bacteria are life threaten agent associated with the misuse and overuse of antibiotics. The biological synthesis of metal oxide nanoparticles is a promising alternative treatment. The current study reported the synthesis of zinc oxide nanoparticles (ZnONPs) using different plants extracts of garlic (), ginger (), and lemon (). The plant extracts not only acts as reducing agents but as stabilizing agents for the produced nanoparticles. Biosynthesized zinc oxide nanoparticles (ZnONPs) were confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM) with energy-dispersive X-ray studies (EDX), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and ultraviolet-visible (UV-vis) spectroscopy. XRD analysis confirmed the production of pure ZnONPs. UV-vis spectroscopy revealed the presence of ZnONPs and reported the characteristic absorption peak of ZnONPs at 370 nm. SEM and TEM confirmed the shape and size of nanoparticles, with an average mean size of 0.3-11.40 nm. This study reported the antibacterial activity and minimum inhibitory concentration of biosynthesized ZnONPs using the broth microdilution method against some clinical pathogenic bacteria. This study also reported the antimicrobial activity of ZnONPs prepared with garlic extract against sp. and sp. While, those prepared with ginger extract were effective against sp. and methicillin-resistant . ZnONPs synthesized by garlic extract were more powerful and effective than ZnONPs synthesized by ginger and lemon extracts.

Supplementary Information: The online version contains supplementary material available at 10.1007/s12088-022-01048-3.