AI Article Synopsis
- A novel LAMP (loop-mediated isothermal amplification) method was developed to detect a significant European forest pathogen that causes economic losses.
- The assay uses DNA sequences from the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene and can amplify the target gene in just 60 minutes at 63°C.
- The method demonstrated high specificity, efficiently detecting the target pathogen in various sample types, making it useful for monitoring imported logs for disease.
Article Abstract
is one of the most aggressive pathogens of forests in Europe, causing considerable economic losses. To detect for disease diagnosis and control, we developed a loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) DNA sequences of . In our study, this LAMP assay was found to be capable of efficiently amplifying the target gene within 60 min at 63°C. In specificity tests, was positively detected, and other species were negative. The detection limit of this assay was found to be 100 pg·μL, and the assay was also successfully tested for use with basidiospore suspensions and wood samples. This study provides a rapid method for diagnosing root and butt rot caused by , which will be of use in port surveillance of logs imported from Europe.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175688 | PMC |
| http://dx.doi.org/10.3389/fcimb.2023.1134921 | DOI Listing |
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