Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic-Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection.

Herein, dendrimer-modified montmorillonite (Mt)-decorated poly-Ɛ-caprolactone (PCL) and chitosan (CHIT)-based nanofibers were prepared. Mt was modified with a poly(amidoamine) generation 1 (PAMAM) dendrimer, and the obtained PAMAM-Mt was incorporated into the PCL-CHIT nanofiber's structure. The PCL-CHIT/PAMAM-Mt nanofibers were conjugated with glutamate oxidase (GluO) to design a bio-based detection system for monosodium glutamate (MSG). PAMAM-Mt was added to the PCL-CHIT backbone to provide a multipoint binding side to immobilize GluO via covalent bonds. After the characterization of PCL-CHIT/PAMAM-Mt/GluO, it was calibrated for MSG. The linear ranges were determined from 0.025 to 0.25 mM MSG using PCL-CHIT/Mt/GluO and from 0.0025 to 0.175 mM MSG using PCL-CHIT/PAMAMG-Mt/GluO (with a detection limit of 7.019 µM for PCL-CHIT/Mt/GluO and 1.045 µM for PCL-CHIT/PAMAMG-Mt/GluO). Finally, PCL-CHIT/PAMAMG-Mt/GluO was applied to analyze MSG content in tomato soup without interfering with the sample matrix, giving a recovery percentage of 103.125%. Hence, the nanofiber modification with dendrimer-intercalated Mt and GluO conjugation onto the formed nanocomposite structures was performed, and the PCL-CHIT/PAMAM-Mt/GluO system was successfully developed for MSG detection.