In recent years, there has been an increased demand for highly sensitive and selective biosensors for neurotransmitters, owing to advancements in science and technology. Real-time sensing is crucial for effective prevention of neurological and cardiovascular diseases. In this review, we summarise the latest progress in aptamer-based biosensor technology, which offers the aforementioned advantages. Our focus is on various biomaterials utilised to ensure the optimal performance and high selectivity of aptamer-based biosensors. Overall, this review aims to further aptamer-based biosensor technology.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136356 | PMC |
http://dx.doi.org/10.3390/bios13040413 | DOI Listing |
Annu Int Conf IEEE Eng Med Biol Soc
July 2024
Biochemical monitoring of sweat through vigilant wearable systems offers new opportunities for improved stress management. A low-power biochemical sensing platform has been developed to perform potentiometric sensing using extended gate field-effect transistors (EGFET) for wearable biochemical monitoring. In vitro validation of the EGFET-enabled electrochemistry was achieved by testing pH and electrolyte concentrations.
View Article and Find Full Text PDFAnnu Int Conf IEEE Eng Med Biol Soc
July 2024
Cytokines, essential regulatory proteins orchestrating various physiological processes and immune responses, play a pivotal role in assessing health status. Conventional cytokine detection methods, such as enzyme-linked immunosorbent assays, are reliable but time-consuming. Electrochemical aptamer-based sensors offer rapid response and higher selectivity, but fall short of achieving non-invasive detection.
View Article and Find Full Text PDFTalanta
August 2025
Department of Pharmacy, Shantou University Medical College, Shantou, Guangdong, 515041, China. Electronic address:
The high-sensitivity detection of low-concentration multicomponent biomarkers in the blood of heart failure (HF) patients using surface-enhanced Raman spectroscopy (SERS) remains a significant challenge. In this study, an ultrasensitive biosensor for the detection of multicomponent HF biomarkers was designed. This biosensor utilizes Au@Ag nanoparticles (Au@Ag NPs) functionalized with Raman reporter molecules (RaRs) as SERS probes, and Ag-coated FeO nanoparticles (FeO-Ag NPs) modified with internal standard (IS) molecules as the capture substrate, offering the dual advantages of magnetic enrichment and SERS enhancement.
View Article and Find Full Text PDFTalanta
August 2025
Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China. Electronic address:
Gliotoxin (GT), a mycotoxin produced by Aspergillus fumigatus, exerts immunosuppressive and pro-apoptotic effects on mammalian cells, posing severe health threat upon human. Ultrasensitively and selectively detecting GT is therefore of great significance. In this work, on the basis of a new GT-specific aptamer, we describe construction of electrochemical label-free biosensor for GT with high sensitivity via exonuclease III (Exo III)-aided dual recycling signal amplification strategy.
View Article and Find Full Text PDFAnal Chem
March 2025
Forensic Research and Development Department, Institute of Environmental Science and Research, P.O. Box 50348, Porirua 5240, New Zealand.
Despite the rapid growth in aptamer-based biosensor research, there remains a significant demand for aptasensors that operate without the need for sample preparation and calibration, to better facilitate real-world applications. Electrochemical aptamer-based (EAB) sensors, particularly those utilizing a dual-frequency, calibration-free approach, have shown promising advances toward commercialization. Single-use, disposable sensors represent a cost-effective solution for at-home and on-site point-of-care (POC) diagnostics.
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