Characterization and bioactivities of : In-silico modeling of as a potential drug source.

In current study the pharmaceutically active herbs was used against coccidiosis, caused by a protozoan: , lead to $ 3 billion loss annually. The aqueous and methanolic extracts of whole plants were applied to assess sporulation inhibition (spi) assay and calculated the inhibitory concentration (IC). For study 9 groups of 14 day old broiler chicks were infected with and three groups were treated different concentrations of methanolic extracts of and post infection. The mean weight gain, oocyst count, diarrhea, biochemical tests, hematology, and histopathology of all groups were analyzed. The herbs were characterized by antioxidant assay, phytochemical screening, Fourier transmission and infrared (FT-IR), Ultra Violet-visible (UV-Vis) spectroscopy and Gas chromatography and mass spectroscopy (GC-MS). The GC-MS identified phyto-compounds of were docked with S-Adenosyl methionine (SAM) synthetase The study revealed that and have minimum IC of 0.14 and 12 mg/ml respectively. The experiment showed that had significantly high anticoccidial potential with significant hematological profile like drug treated controls. The histology of treated chicks also showed recovery in the studied tissues. The antioxidant assay showed that have 4.19U/mg Superoxide dismutase (SOD) and 33.96 µM/mg Glutathione (GSH) quantities The chemical characterization confirmed the presence of large number of organic compounds, however Flavonoids found only in , which suggests the anticoccidial potential of because flavonoids as antagonist of thiamine (Prinzo, 1999), because it promotes the carbohydrate synthesis required. Strychane, 1-acetyl-20a-hydroxy-16-methylene has best binding of with target protein with lowest binding score (-6.4 Kcal/mol), suggests its anticoccidial potential in poultry.