AI Article Synopsis
- Recent studies highlight that poor air quality, particularly due to human activities, leads to harmful health effects, including higher rates of respiratory diseases and complications from COVID-19.
- The research aims to investigate how exposure to coarse particulate matter (PM10) influences the inflammatory response and viral replication in cells infected with SARS-CoV-2.
- Findings indicate that PM10 exposure increases the production of various inflammatory cytokines and boosts SARS-CoV-2 replication, suggesting that air pollution may contribute to more severe COVID-19 outcomes.
Article Abstract
Introduction: In the last decades, a decrease in air quality has been observed, mainly associated with anthropogenic activities. Air pollutants, including particulate matter (PM), have been associated with adverse effects on human health, such as exacerbation of respiratory diseases and infections. High levels of PM in the air have recently been associated with increased morbidity and mortality of COVID-19 in some regions of the world.
Objective: To evaluate the effect of coarse particulate matter (PM10) on the inflammatory response and viral replication triggered by SARS-CoV-2 using models.
Methods: Peripheral blood mononuclear cells (PBMC) from healthy donors were treated with PM10 and subsequently exposed to SARS-CoV-2 (D614G strain, MOI 0.1). The production of pro-inflammatory cytokines and antiviral factors was quantified by qPCR and ELISA. In addition, using the A549 cell line, previously exposed to PM, the viral replication was evaluated by qPCR and plaque assay.
Results: SARS-CoV-2 stimulation increased the production of pro-inflammatory cytokines in PBMC, such as IL-1β, IL-6 and IL-8, but not antiviral factors. Likewise, PM10 induced significant production of IL-6 in PBMCs stimulated with SARS-CoV-2 and decreased the expression of OAS and PKR. Additionally, PM10 induces the release of IL-1β in PBMC exposed to SARS-CoV-2 as well as in a co-culture of epithelial cells and PBMCs. Finally, increased viral replication of SARS-CoV-2 was shown in response to PM10.
Conclusion: Exposure to coarse particulate matter increases the production of pro-inflammatory cytokines, such as IL-1β and IL-6, and may alter the expression of antiviral factors, which are relevant for the immune response to SARS-CoV-2. These results suggest that pre-exposure to air particulate matter could have a modest role in the higher production of cytokines and viral replication during COVID-19, which eventually could contribute to severe clinical outcomes.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166799 | PMC |
| http://dx.doi.org/10.3389/fimmu.2023.1161135 | DOI Listing |
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