In photosynthetic green sulfur bacteria, the electron transfer reaction from menaquinol:cytochrome oxidoreductase to the P840 reaction center (RC) complex occurs directly without any involvement of soluble electron carrier protein(s). X-ray crystallography has determined the three-dimensional structures of the soluble domains of the gene product and Rieske iron-sulfur protein (ISP). The former is a mono-heme cytochrome with an α-absorption peak at 556 nm. The overall fold of the soluble domain of cytochrome -556 (designated as cyt -556) consists of four α-helices and is very similar to that of water-soluble cyt -554 that independently functions as an electron donor to the P840 RC complex. However, the latter's remarkably long and flexible loop between the α3 and α4 helices seems to make it impossible to be a substitute for the former. The structure of the soluble domain of the Rieske ISP (Rieske protein) shows a typical β-sheets-dominated fold with a small cluster-binding and a large subdomain. The architecture of the Rieske protein is bilobal and belongs to those of -type Rieske ISPs. Nuclear magnetic resonance (NMR) measurements revealed weak non-polar but specific interaction sites on Rieske protein when mixed with cyt -556. Therefore, menaquinol:cytochrome oxidoreductase in green sulfur bacteria features a Rieske/cyt complex tightly associated with membrane-anchored cyt -556.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172866 | PMC |
| http://dx.doi.org/10.1016/j.crstbi.2023.100101 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Propofol Triggers Cell Death in Lung Cancer Cells by Increasing PANX1 Expression, Activating the Mitochondrial Cell Death Pathway, and Enhancing ROS Levels.
Discov Med
November 2024
Anesthesiology and Perioperative Medicine Department, Shandong Public Health Clinical Center, 250000 Jinan, Shandong, China.
Background: Lung cancer treatment remains a global challenge due to tumor cell resistance. Propofol, traditionally used as an anesthetic, has demonstrated potential anti-tumor properties. This study seeks to elucidate how propofol induces cell death in lung cancer cells by upregulating Pannexin 1 (PANX1) expression, activating the mitochondrial cell death pathway, and augmenting reactive oxygen species (ROS) production.
View Article and Find Full Text PDFThe Effect of Pomegranate Peel Extract on the Oxidative and Inflammatory Status in the Spleens of Rats with Metabolic Syndrome.
Int J Mol Sci
November 2024
Division of Clinical Chemistry and Laboratory Haematology, Department of Medical Laboratory Diagnostics, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A, 50-556 Wroclaw, Poland.
Polyphenols have antioxidant and anti-inflammatory properties and maintain the immune system in balance; therefore, the aim of the study was to investigate the effect of polyphenols present in pomegranate peel extract on the spleens of rats with metabolic syndrome. The study objects were adult male Zucker Diabetic Fatty (ZDF-Leprfa/Crl, fa/fa) rats. The rats were divided into a control group (MetS) consisting of rats with metabolic syndrome and four study groups consisting of rats with metabolic syndrome (MetS + 100 mg and MetS + 200 mg) or healthy animals (H + 100 mg and H + 200 mg) receiving polyphenol extract at a dose of 100 mg or 200 mg/kg, respectively.
View Article and Find Full Text PDFAdiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis.
In Vitro Cell Dev Biol Anim
August 2024
Tianjin Key Laboratory of Animal Molecular Breeding and BiotechnologyTianjin Engineering Research Center of Animal Healthy FarmingInstitute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.
Adiponectin has previously been investigated for exerting its protective effect against myocardial injury through anti-apoptotic and anti-oxidative actions. Therefore, the present study aimed to investigate the nature and mechanism of adiponectin inhibition of HO-induced apoptosis in chicken skeletal myoblasts. Skeletal muscle satellite cells were differentiated and assigned into three groups.
View Article and Find Full Text PDFSoluble domains of cytochrome -556 and Rieske iron-sulfur protein from m: Crystal structures and interaction analysis.
Curr Res Struct Biol
April 2023
Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan.
In photosynthetic green sulfur bacteria, the electron transfer reaction from menaquinol:cytochrome oxidoreductase to the P840 reaction center (RC) complex occurs directly without any involvement of soluble electron carrier protein(s). X-ray crystallography has determined the three-dimensional structures of the soluble domains of the gene product and Rieske iron-sulfur protein (ISP). The former is a mono-heme cytochrome with an α-absorption peak at 556 nm.
View Article and Find Full Text PDFBiomolecular interactions of lysosomotropic surfactants with cytochrome c and its effect on the protein conformation: A biophysical approach.
Int J Biol Macromol
April 2019
Department of Inorganic Chemistry, Faculty of Pharmacy, Wroclaw Medical University, 50-556 Wrocław, Poland.
The molecular interactions between two single-chain lysosomotropic surfactants DMM-11 (2-Dodecanoyloxyethyl)trimethylammonium bromide) and DMPM-11 (2-Dodecanoyloxypropyl)trimethylammonium bromide) with a small heme-protein (cytochrome c (cyt-c)) in Hepes buffer (pH = 7.4) were extensively investigated by surface tension, dynamic light scattering (DLS), circular dichroism (CD) and fluorescence spectroscopy in combination with molecular dynamic simulation techniques. The results demonstrated that surfactants can destroy the hydrophobic cavity of cyt-c, make the α-helical become loose and convert it into the β-sheet structure.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!