AI Article Synopsis
- Spinal cord injury (SCI) leads to significant disabilities, and researchers are exploring the therapeutic potential of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) to promote recovery.
- This study details an effective method for producing cytochalasin B-induced EVs through vortex mixing and filtration, aimed at enhancing their therapeutic use.
- Results from testing repeated intrathecal injections of autologous MSC-derived EVs in pigs with contused SCI show promise in improving locomotor activity by supporting axon remyelination and nerve tissue reperfusion.
Article Abstract
Spinal cord injury (SCI) remains one of the current medical and social problems, as it causes deep disability in patients. The use of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) is one strategy for stimulating the post-traumatic recovery of the structure and function of the spinal cord. Here, we chose an optimal method for obtaining cytochalasin B-induced EVs, including steps with active vortex mixing for 60 s and subsequent filtration to remove nuclei and disorganized inclusions. The therapeutic potential of repeated intrathecal injection of autologous MSC-derived EVs in the subacute period of pig contused SCI was also evaluated for the first time. In this study, we observed the partial restoration of locomotor activity by stimulating the remyelination of axons and timely reperfusion of nervous tissue.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10179045 | PMC |
| http://dx.doi.org/10.3390/ijms24098240 | DOI Listing |
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