HPTLC Analysis and Chemical Composition of Selected Essential Oils.

Molecules

United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Subtropical Horticulture Research Station (SHRS), Miami, FL 33158, USA.

Published: May 2023

Tea tree oil (TTO) is a volatile essential oil obtained by distillation, mainly from the Australian native plant (Maiden & Betche) Cheel (Myrtaceae). In this study, a comparative analysis of the chemical constituents of seven tea tree oils () and four other spp. oils (, (M), two chemotypes of , (M and M), and (M)) was carried out using gas chromatography-mass spectrometry (GC-MS) and high-performance thin-layer chromatography (HPTLC). Among the seven TTOs, terpinen-4-ol (37.66-44.28%), γ-terpinene (16.42-20.75%), α-terpinene (3.47-12.62%), α-terpineol (3.11-4.66%), and terpinolene (2.75-4.19%) were the most abundant compounds. On the other hand, the most abundant compounds of the other oils varied, such as 1,8-cineole (64.63%) in M oil, (E)-nerolidol (48.40%) and linalool (33.30%) in M oil, 1,8-cineole (52.20%) in M oil, and linalool (38.19%) and 1,8-cineole (27.57%) in M oil. HPTLC fingerprinting of oils enabled the discrimination of TTO oils from other spp. oils. Variation was observed in the profile of the R values among EOs. The present study shows that HPTLC is one of the best ways to identify and evaluate the quality control in authenticating TTOs, other EOs, or EOs from other species within the Myrtaceae.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10180325PMC
http://dx.doi.org/10.3390/molecules28093925DOI Listing

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