sp. nov., a moderately halophilic bacterium isolated from sea tidal flat environment.

Two moderately halotolerant bacterium strains, designated PJ-16 and PJ-38, were isolated from a tidal flat of the red beach in Panjin City, Liaoning Province, PR China. Cells were found to be Gram-stain-negative, aerobic, motile, rod-shaped with a single polar flagellum. Optimum growth of strain PJ-16 occurred at 30 °C, pH 7.0 and 0.2-8.0  % (w/v) NaCl, and strain PJ-38 at 30 °C, pH 6.0-7.0 and 0.2-8.0  % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PJ-16 was most closely related to KCTC 62941 (99.2 % 16S rRNA gene sequence similarity), DSM 16394 (98.6 %), JCM 19399 (98.4 %) and KCTC 42705 (98.2 %), and strain PJ-38 was most closely related to KCTC 62941 (99.1 %), DSM 16394 (98.6 %), JCM 19399 (98.4 %) and KCTC 42705 (98.1 %). The G+C content of the genomic DNA of strain PJ-16 based on its draft genomic sequence was 57.4 mol%. The major cellular fatty acids of strain PJ-16 were C, C 7/C 6 and C 9. The major respiratory quinone of PJ-16 was ubiquinone-9 and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The results of the phenotypic, phylogenetic and genomic analyses revealed that strains PJ-16 and PJ-38 represent a novel species of the genus , and the name sp. nov. is proposed. The type strain is PJ-16 (= CGMCC 1.13694= KCTC 72023).