AI Article Synopsis
- - The text outlines procedures for creating in vitro assays to monitor the activity of papain-like protease in coronaviruses, as well as a cell-based immunofluorescence assay for infection.
- - These assays can be adapted for high-throughput screening, allowing researchers to evaluate the effectiveness of new protease inhibitors against coronaviruses and other viruses.
- - Additionally, the cell-based immunofluorescence assay enables visual analysis of how well new antiviral compounds work, with comprehensive details available in the reference to Jeong et al. (2022).
Article Abstract
Here, we describe detailed steps to constitute an in vitro assay for monitoring papain-like protease of coronavirus and a cell-based immunofluorescence infection assay. These assays can be adapted for high-throughput screening to determine the efficacy of novel protease inhibitors of coronaviruses and other viruses. In addition, cell-based immunofluorescence infection assay can be used to visually analyze antiviral efficacy of any novel compounds. For complete details on the use and execution of this protocol, please refer to Jeong et al. (2022)..
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123355 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102295 | DOI Listing |
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