AI Article Synopsis
- DNA origami is a flexible nanoengineering tool used in fields like membrane physiology and biophysics, allowing for easy modification of DNA strands to create membrane-active devices.
- Biological membranes are crucial for cell structure and function, making them prime targets for DNA origami in synthetic biology and biomedical research.
- The chapter outlines experimental methods to study how DNA origami interacts with synthetic membrane models, providing protocols for creating lipid model membranes and using microscopy to characterize these interactions.
Article Abstract
DNA origami is an extremely versatile nanoengineering tool with widespread applicability in various fields of research, including membrane physiology and biophysics. The possibility to easily modify DNA strands with lipophilic moieties enabled the recent development of a variety of membrane-active DNA origami devices. Biological membranes, as the core barriers of the cells, display vital structural and functional roles. Therefore, lipid bilayers are widely popular targets of DNA origami nanotechnology for synthetic biology and biomedical applications. In this chapter, we summarize the typical experimental methods used to investigate the interaction of DNA origami with synthetic membrane models. Herein, we present detailed protocols for the production of lipid model membranes and characterization of membrane-targeted DNA origami nanostructures using different microscopy approaches.
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| http://dx.doi.org/10.1007/978-1-0716-3028-0_14 | DOI Listing |
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