A fluorescent probe Y((1,1'-([1,1'-biphenyl]-4,4'-diylbis(3-(2-hydroxyphenyl)-4,5-dihydro-1H-pyrazole-5,1-diyl)) bis(ethan-1-one))) was designed and synthesized, which could be used to Cu and Fe sensors. Through the study of optical properties, the probe Y shows good selectivity and sensitivity to Cu and Fe in aqueous tetrahydrofuran solution [10.0 mM HEPES, pH 7.4, THF-HO = 9:1(v/v)] with has excellent anti-interference performance, and its detection limits were 0.931 uΜ for Cu and 0.401uΜ for Fe. The coordination mechanism of probe Y with Cu and Fe was speculated and verified at DFT level and HRNM. By Hela cytotoxicity and imaging tests, probe Y not only has good biocompatibility, but also can be used for sensing Cu in cells.
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