The prognostic significance and immune correlation of SLC10A3 in low-grade gliomas revealed by bioinformatic analysis and multiple immunohistochemistry.

Purpose: Despite the fact that genetic risk factors contribute to low-grade gliomas (LGGs), the role of critical genes as prognostic and theraputic biomarkers is quite limited. This study is designed to comprehensively investigate the prognostic role and predictive ability of solute carrier family 10 member 3 () for immunotherapy in LGGs.

Methods: We analyzed the prognostic value of from multiple datasets of LGG patients, and explored its immune correlation via multiple algorithms. Finally, we independently confirmed the clinical significance and its immune correlation using the multiplex staining assay of LGG samples on the tissue microarray.

Results: mRNA was up-regulated in LGGs compared with normal brain tissues, and correlated with tumor grade, histological type, IDH wide type and non-codel 1p19q. Up-regulation of SLC10A3 transcription was remarkably associated with shortened overall survival time compared with down-regulation in TCGA, CGGA and Rembrandt datasets, and exhibited good predictive ability for survival outcomes among LGGs. Correlation analyses showed that mRNA expression correlates well with the six immune check points and immune cells. When the expression and immune correlation of at the translational level were verified via multiplex immunohistochemistry, expression of protein was higher in LGG compared with normal tissues, and expression of protein was correlated well with macrophage, CD4 + T cell and B cell.

Conclusions: Up-regulation of mRNA is statistically associated with adverse survival outcomes and immune infiltration among LGGs. might be a reliable survival predictor and a promising immunotherapy target for LGG patients.