Type 2C protein phosphatases (PP2Cs) play key roles in regulating plant senescence and ripening. Here, we discovered a subfamily F PP2C, SlPP2C, associated with leaf senescence through transcriptome analysis. The gene expression, protein accumulation, and promoter activity of SlPP2C all gradually increased along with the progression of leaf and flower senescence and fruit ripening in tomato. Also, the expression of SlPP2C was highly up-regulated by the senescent-inducible hormone treatments, showing more sensitivity to ethylene (ETH) and abscisic acid (ABA). Meanwhile, SlPP2C RNA interference (RNAi) tomato transgenic lines showed obvious delayed senescence and ripening phenotypes of leaves, flowers, and fruits. SlPP2C RNAi lines delayed leaf senescence with higher chlorophyll fluorescence and content, and lower expressions of senescence marker genes (SlSGR1 and SlSAG12) compared to WT. SlPP2C RNAi lines clearly delayed flower senescence time; it was at least twice longer than WT. SlPP2C RNAi lines delayed fruit ripening, exhibiting higher firmness and lower polygalacturonase activity compared to WT. In addition, we proved that SlPP2C is unable to interact with any of the ABA receptors (SlPYLs). Together, the results demonstrate that SlPP2C is a senescence-related and ripening-related gene.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/ppl.13925 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transcription factors SlNOR and SlNOR-like1 regulate steroidal glycoalkaloids biosynthesis in tomato fruit.
Int J Biol Macromol
January 2025
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address:
Steroidal glycoalkaloids (SGAs) are specialized metabolites in Solanaceae that serve as defensive compounds and undergo significant compositional changes during fruit ripening. This study explored the roles of transcription factors SlNOR and SlNOR-like1 in SGAs biosynthesis during tomato fruit development. UPLC-MS/MS revealed dynamic changes in four major SGAs: tomatidine, β-tomatine, α-tomatine, and Esculeoside A.
View Article and Find Full Text PDFThe time is ripe: Natural variability of MdNAC18.1 promoter plays a major role in fruit ripening.
Plant Cell
December 2024
Assistant Features Editor, The Plant Cell, American Society of Plant Biologists.
Unveiling the Potential of Southern Elderberry (Sambucus australis): Characterization of Physicochemical Properties, Carbohydrates, Organic Acids and Biophenols.
Plant Foods Hum Nutr
January 2025
Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz 2290 (C1425FQB), Buenos Aires, CABA, República Argentina.
Sambucus australis is a wild species with purple fruits like berries. It is native from South America and can be found in Argentina, Uruguay, Paraguay, Bolivia, and Brazil. A comprehensive characterization of S.
View Article and Find Full Text PDFQualitative and Quantitative Analyses of 1-Aminocyclopropane-1-carboxylic Acid Concentrations in Plants Organs Using Phenyl Isothiocyanate Derivatization.
J Agric Food Chem
January 2025
Engineering Research Center of Protection and Utilization of Plant Resources, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning Province 110866, China.
1-Aminocyclopropane-1-carboxylic acid (ACC) is a direct precursor of phytohormone ethylene. We used a phenyl isothiocyanate (PITC) derivatization modification method combined with spectrographic analysis to isolate and identify three products of the derivatization reactions of ACC and PITC. The MRM mode of UPLC-MS/MS was used to establish the analysis of 6-phenyl-5-thioxo-4,6-diazaspiro[2.
View Article and Find Full Text PDFSubtilases: a major prospect to the genome editing in horticultural crops.
Front Plant Sci
January 2025
Institute of Life Sciences, Kangwon National University, Chuncheon, Republic of Korea.
Plant peptides, synthesized from larger precursor proteins, often undergo proteolytic cleavage and post-translational modifications to form active peptide hormones. This process involves several proteolytic enzymes (proteases). Among these, SBTs (serine proteases) are a major class of proteolytic enzymes in plants and play key roles in various regulatory mechanisms, including plant immune response, fruit development and ripening, modulating root growth, seed development and germination, and organ abscission.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!