Identification of a Novel Allele Conferring a Wide Spectrum of Resistance to Powdery Mildew in Wheat Accession PI 351817.

Phytopathology

U.S. Department of Agriculture-Agricultural Research Service, Hard Winter Wheat Genetics Research Unit, Manhattan, KS 66506.

Published: October 2023

Powdery mildew is caused by the highly adaptive biotrophic fungus f. sp. infecting wheat worldwide. Novel powdery mildew resistance genes are urgently needed that can be used rapidly in wheat cultivar development with minimal disruption of trait advances elsewhere. PI 351817 is a German cultivar exhibiting a wide spectrum of resistance to f. sp. isolates collected from different wheat-growing regions of the United States. Evaluation of an F population and 237 F lines derived from OK1059060-2C14 × PI 351817 for responses to f. sp. isolate --- identified a single dominant gene, designated , for powdery mildew resistance in PI 351817. Using bulked segregant analysis (BSA) and simple sequence repeat (SSR) markers, was mapped in the terminal region of the long arm of chromosome 2A. Deep sequencing of the genotyping-by-sequencing libraries of the two parental lines identified a set of single-nucleotide polymorphism (SNP) markers in the 2AL candidate gene region. Those SNP markers was subsequently converted to Kompetitive allele-specific PCR (KASP) markers for genotyping the mapping population. Linkage analysis delimited to a 634-kb interval between Stars-KASP656 (771,207,512 bp) and Stars-KASP662 (771,841,609 bp) on 2AL, based on the Chinese Spring reference sequence IWGSC RefSeq v 2.1. Tests of allelism indicated that is located at the locus. shows resistance to all f. sp. isolates used in this study and can be used to enhance powdery mildew resistance in the United States. KASP markers flanking can be used to select in wheat breeding programs after further tests for polymorphism.

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Source
http://dx.doi.org/10.1094/PHYTO-01-23-0032-RDOI Listing

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