Isolated lamb hearts were perfused for eight hours at 38 degrees with stroma-free hemoglobin solution (SFHS). The preservation of cardiac structure and function was studied. Control hearts perfused with blood (N = 6) developed no ventricular failure or significant weight gain (13% +/- 5), showed no alteration of cellular ultrastructure, and little interstitial edema. Hearts perfused with 7% (N = 7) or 11% (N = 5) SFHS contracted less well, became edematous (22% and 44% weight gain) and showed altered mitochondria, capillary endothelial swelling and hemoglobin extravasation into the interstitial space. The addition of 5-7% albumin to SFHS (N = 9) markedly reduced interstitial edema (weight gain 11% +/- 13), preserved mitochondria, prevented endothelial swelling, and limited transcapillary escape of hemoglobin. Thus isolated hearts perfused with SFHS develop vascular endothelial damage and an increase in capillary permeability. The addition of plasma proteins to the perfusate protects against this injury.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1397097 | PMC |
| http://dx.doi.org/10.1097/00000658-197903000-00013 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Compartmental modeling for blood flow quantification from dynamic O-water PET images of humans: a systematic review.
Ann Nucl Med
January 2025
Turku PET Centre, University of Turku and Turku University Hospital, Turku, Finland.
Dynamic positron emission tomography (PET) can be used to non-invasively estimate the blood flow of different organs via compartmental modeling. Out of different PET tracers, water labeled with the radioactive O isotope of oxygen (half-life of 2.04 min) is freely diffusable, and therefore, very well-suited for blood flow quantification.
View Article and Find Full Text PDFOn the Effect of the Patient Table on Attenuation in Myocardial Perfusion Imaging SPECT.
EJNMMI Phys
January 2025
Institute of Radiology, Nuclear Medicine and Molecular Imaging, Heart and Diabetes Center North Rhine-Westphalia, University Hospital (Ruhr University Bochum), Medical Faculty OWL (Bielefeld University), Bad Oeynhausen, Germany.
Background: The topic of the effect of the patient table on attenuation in myocardial perfusion imaging (MPI) SPECT is gaining new relevance due to deep learning methods. Existing studies on this effect are old, rare and only consider phantom measurements, not patient studies. This study investigates the effect of the patient table on attenuation based on the difference between reconstructions of phantom scans and polar maps of patient studies.
View Article and Find Full Text PDFExtracellular vesicle-mediated VEGF-A mRNA delivery rescues ischaemic injury with low immunogenicity.
Eur Heart J
January 2025
School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, 2199 Lishui Rd, Nanshan, Shenzhen, Guangdong Province 518055, China.
Background And Aims: Lackluster results from recently completed gene therapy clinical trials of VEGF-A delivered by viral vectors have heightened the need to develop alternative delivery strategies. This study aims to demonstrate the pre-clinical efficacy and safety of extracellular vesicles (EVs) loaded with VEGF-A mRNA for the treatment of ischaemic vascular disease.
Methods: After encapsulation of full-length VEGF-A mRNA into fibroblast-derived EVs via cellular nanoporation (CNP), collected VEGF-A EVs were delivered into mouse models of ischaemic injury.
Systemic Circulation in Advanced Heart Failure and Cardiogenic Shock: State-of-the-Art Review.
Circ Heart Fail
January 2025
The CardioVascular Center, Tufts Medical Center, Boston, MA (S.L.H., K.D.E., G.G., N.K.K.).
The integrative physiology of the left ventricle and systemic circulation is fundamental to our understanding of advanced heart failure and cardiogenic shock. In simplest terms, any increase in aortic stiffness increases the vascular afterload presented to the failing left ventricle. The net effect is increased myocardial oxygen demand and reduced coronary perfusion pressure, thereby further deteriorating contractile function.
View Article and Find Full Text PDFNon-invasive [O]HO PET measurements of cerebral perfusion and cerebrovascular reactivity using an additional heart scan.
J Cereb Blood Flow Metab
January 2025
Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital Bispebjerg and Frederiksberg, Copenhagen, Denmark.
Obtaining the arterial input function (AIF) is essential for quantitative regional cerebral perfusion (rCBF) measurements using [O]HO PET. However, arterial blood sampling is invasive and complicates the scanning procedure. We propose a new non-invasive dual scan technique with an image derived input function (IDIF) from an additional heart scan.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!