AI Article Synopsis
- Obesity is linked to higher mortality rates in ovarian cancer, with leptin, a hormone produced by fat tissue, playing a significant role in cancer development.
- The study explored how varying levels of leptin influence the growth of ovarian cancer cells (specifically OVCAR-3 and MDAH-2774), assessing cell viability and cytokine expression changes.
- Results showed that leptin promotes cell proliferation in both cancer lines, affecting various cytokines differently; for instance, IL-1 levels increased in OVCAR-3, while TGF-β increased in MDAH-2774 after leptin treatment.
Article Abstract
Background: Obesity is associated with increased mortality among ovarian cancer and is a poor prognostic factor. There are significant links between the leptin hormone, a product of the obesity gene, and the development of ovarian cancer. Leptin is a vital hormone-like cytokine secreted from adipose tissue and is mainly involved in the maintenance of energy homeostasis. It regulates several intracellular signaling pathways and also interacts with various hormones and energy regulators. It acts as a growth factor by stimulating cell proliferation and differentiation and in this way contributes to cancer cell development. The aim of the study was to investigate the effects of leptin on human ovarian cancer cells.
Methods: In this study, the effects of increasing the concentration of leptin were investigated on the cell viability of OVCAR-3 and MDAH-2774 ovarian cancer lines by MTT assay. Moreover, to elucidate the molecular mechanisms of leptin in ovarian cancer cells, changes in the expression levels of 80 cytokines were evaluated after leptin treatment a human cytokine antibody array.
Results: Leptin increases the proliferation of both ovarian cancer cell lines. IL-1 level was increased in OVCAR-3 cells and TGF-β level was increased in MDAH-2774 cells after leptin treatment. A decrease in IL-2, MCP-2/CCL8 and MCP-3/CCL7 levels was detected in both ovarian cancer cell lines with leptin administration. An increase in IL-3 and IL-10 expressions, insulin-like growth factor binding proteins (IGFBP) IGFBP-1, IGFBP-2 and IGFBP-3 levels were detected in both ovarian cancer cell lines with leptin administration. In conclusion; leptin has a proliferative effect on human ovarian cancer cell lines and affects different cytokines in different types of ovarian cancer cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10122840 | PMC |
| http://dx.doi.org/10.7717/peerj.15246 | DOI Listing |
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