Anti-biofilm and anti-inflammatory active diterpene isolated from the fruit of R.E.Fr.

(Annonaceae) is a plant with limited phytochemical and pharmacological evidence. Thus, using LC-MS/MS, we performed exploratory analyses of the fruit extract of , resulting in the tentative identification of alkaloids () and diterpenes (). Through the application of chromatography techniques with the extract of two kaurane diterpenes were isolated, xylopinic acid () and -15-oxo-kaur-16-en-19-oic acid (). Their structures were established using spectroscopy (NMR 1D/2D) and mass spectrometry. The isolated compounds were submitted to anti-biofilm analysis against , anti-neuroinflammatory and cytotoxic activity in BV-2 cells. Compound (201.75 µM) inhibited 35% of bacterial biofilm formation and high anti-inflammatory activity in BV-2 (IC = 0.78 µM). In conclusion, the results demonstrated that compound was characterized for the first time with pharmacological potential in the development of new alternatives for studies with neuroinflammatory diseases.