[Construction of fluorescent transgenic zebrafish Tg (.: EGFP)].

In order to develop a transgenic zebrafish line with green fluorescent protein (enhanced green fluorescent protein, EGFP) expressed specifically in muscle and heart, the recombinant expression vector constructed using the zebrafish . gene promoter fragment and EGFP gene coding sequence and the capped mRNA of transposase were co-injected into the zebrafish 1-cell stage embryos. The stable genetic Tg (.: EGFP) transgenic zebrafish line was successfully developed by fluorescence detection, followed by genetic hybridization screening and molecular identification. Fluorescence signals and whole-mount hybridization showed that EGFP expression was located in muscle and heart, the specificity of which was consistent with the expression of . mRNA. Inverse PCR showed that EGFP was integrated into chromosomes 4 and 11 of zebrafish in No. 33 transgenic line, while integrated into chromosome 1 in No. 34 transgenic line. The successful construction of this fluorescent transgenic zebrafish line, Tg (.: EGFP), laid a foundation for the research of muscle and heart development and related diseases. In addition, the transgenic zebrafish lines with strong green fluorescence can also be used as a new ornamental fish.