Overexpression of Krüppel like factor 2 (Klf2) or Klf7 inhibits adipocyte formation. However, it remains unclear whether Klf2 regulates expression in adipose tissue. In this study, oil red O staining and Western blotting were employed to study the effect of Klf2 overexpression on the differentiation of chicken preadipocytes. The results showed that Klf2 overexpression inhibited the differentiation of chicken preadipocytes induced by oleate and the expression of , while promoted expression in chicken preadipocytes. Spearman correlation analysis was used to study the correlation between the expression data of and in the adipose tissue of both human and chicken. The results showed that there was a significantly positive correlation between the expression of and in adipose tissues (r > 0.1). Luciferase reporter assay showed that overexpression of Klf2 significantly promoted the activity of chicken promoter (-241/-91, -521/-91, -1 845/-91, -2 286/-91, -1 215/-91; < 0.05). In addition, the activity of promoter (-241/-91) reporter in chicken preadipocytes was significantly positively correlated with the amount of overexpression plasmid transfected (=0.917 66, =1.074×10). Moreover, Klf2 overexpression significantly promoted the mRNA expression of in chicken preadipocytes ( < 0.05). In conclusion, upregulation of expression might be one of the pathways that Klf2 inhibits chicken adipocyte differentiation, and the sequence from -241 bp to -91 bp upstream chicken translation start site might mediate the regulation of Klf2 on transcription.
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http://dx.doi.org/10.13345/j.cjb.220554 | DOI Listing |
Int J Biol Macromol
December 2024
The Shennong Laboratory, Henan Agricultural University, Zhengzhou, Henan Province 450046, China. Electronic address:
Non-coding RNA is known to play a crucial role in the generation and deposition of intramuscular fat (IMF) in vertebrates by regulating the expression of genes involved in the synthesis, degradation and transportation of IMF. With the introduction of the competing endogenous RNA (ceRNA) hypothesis, circular RNA (circRNA) and long non-coding RNA (lncRNA) have been identified as natural "sponges" for microRNA (miRNA), yet their precise mechanisms and biological functions in chicken IMF are still not fully understood. In earlier research, we observed a significant association between Carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) and fatty acid metabolism in chicken breast muscle.
View Article and Find Full Text PDFPoult Sci
January 2025
USDA-ARS, Plant Genetics Research Unit, Columbia, MO 65211, USA. Electronic address:
In the broiler industry, intensive genetic selection has been placed on muscle growth which has undesirably led to increased fat accretion. Models of chicken preadipocyte differentiation in vitro have conventionally used incubators without the ability to control oxygen (O) tension; thus, the cells are exposed to atmospheric (∼20-21%) O, which is supraphysiological compared to the O tension within adipose tissue. The objective of this study was to investigate embryonic broiler preadipocyte differentiation at different O tensions, including atmospheric (20%), physiological (5%), and hypoxic (1%).
View Article and Find Full Text PDFJ Agric Food Chem
November 2024
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
The excessive deposition of abdominal fat tissue (AFT) in broilers has emerged as a major concern in the poultry industry. Despite some progress in recent years, the molecular mechanisms underlying AFT development remain ambiguous. The current study combined RNA-seq with transposase-accessible chromatin sequencing (ATAC-seq) to map the dynamic profiling of chromatin accessibility and transcriptional reprogramming in AFT adipocyte differentiation in broilers at day 3 (D3) and D14.
View Article and Find Full Text PDFNat Commun
October 2024
College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, PR China.
Chickens are the most abundant agricultural animals globally, with controlling abdominal fat deposition being a key objective in poultry breeding. While GWAS can identify genetic variants associated with abdominal fat deposition, the precise roles and mechanisms of these variants remain largely unclear. Here, we use male chickens from two lines divergently selected for abdominal fat deposition as experimental models.
View Article and Find Full Text PDFPoult Sci
December 2024
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou 450046, China. Electronic address:
Though it is well known that insulin-like growth factor (IGF) binding protein 7 (IGFBP7) plays an important role in myogenesis and adipogenesis in mammals, its impact on the proliferation, differentiation, and lipid deposition in chicken primary myoblasts (CPM) and intramuscular preadipocytes remains unexplored. In the present study, we firstly examined the correlation between SNPs within the genomic sequence of the IGFBP7 gene and carcass and blood chemical traits in a F2 resource population by genetic association analysis, and found that a significant correlation between the SNP (4_49499525) located in the intron region of IGFBP7 and serum high-density lipoproteins (HDL). We then examined the expression patterns of IGFBP7 across different stages of proliferation and differentiation in CPMs and intramuscular preadipocytes via qPCR, and explored the biological functions of IGFBP7 through gain- and loss-of-function experiments and a range of techniques including qPCR, CCK-8, EdU, flow cytometry, Western blot, immunofluorescence, and Oil Red O staining to detect the proliferation, differentiation, and lipid deposition in CPMs and intramuscular preadipocytes.
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