Determination of Chemical Composition and Antimicrobial Activity of the CO Extract of L.

The article presents parameters for obtaining a carbon dioxide extract from the subterranean part of that contains a valuable set of organic substances and has a certain antimicrobial effect. . Raw materials were collected in the Almaty region (Republic of Kazakhstan). The CO extract of herbs was obtained under subcritical conditions. A gas chromatograph with a mass spectrometric detector was used to determine the compositional breakdown of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilutions and the disk-diffusion method. Three microbial test strains were used: ATCC 6538-P, ATCC 8739, and ATCC 10231. . To extract biologically active substances from the subterranean part of L., we have chosen carbon dioxide extraction technology, a technology for processing carbon dioxide (CO) raw materials, which allows us to extract various substances in high concentrations. Carbon dioxide extraction technology is an effective and environmentally safe way to isolate various biologically active substances contained in medicinal plant raw materials. In the composition of the CO extract of L. 43 components were identified, the main of which are -linolenic acid, 8.30%; myristic acid, 6.40%; caryophyllene, 6.92%; spatulous, 6.62%; and other main identified compounds and their percentage. . The study showed that the CO extract of L. contains biologically active compounds that have a pronounced antimicrobial effect against clinically significant microorganisms, such as and .