AI Article Synopsis
- Piscidins are important for the fish immune response, helping to fight off foreign microbes and maintain immune balance.
- Two specific antimicrobial peptides, LjPL-3 and LjPL-2, were studied in Japanese sea bass, showing different levels of expression in various tissues after bacterial infection.
- Both peptides boosted the immune response by reducing inflammation and enhancing monocyte/macrophage activity, with LjPL-2 being particularly effective in killing bacteria, ultimately helping the fish survive infections better.
Article Abstract
Piscidins participate in the innate immune response of fish, which aims to eliminate recognized foreign microbes and restore the homeostasis of immune system. We characterized two piscidin-like antimicrobial peptides (LjPL-3 and LjPL-2) isolated from Japanese sea bass (Lateolabrax japonicus). LjPL-3 and LjPL-2 showed different expression patterns in tissues. After Vibrio harveyi infection, the mRNA expression of LjPL-3 and LjPL-2 was upregulated in the liver, spleen, head kidney, and trunk kidney. The synthetic mature peptides LjPL-3 and LjPL-2 exhibited different antimicrobial spectra. Furthermore, LjPL-3 and LjPL-2 treatments decreased inflammatory cytokine production while promoting chemotaxis and phagocytosis in monocytes/macrophages (MO/MФ). LjPL-2, but not LjPL-3, displayed bacterial killing capability in MO/MФ. LjPL-3 and LjPL-2 administration increased Japanese sea bass survival after V. harveyi challenge, which was accompanied by a decline in bacterial burden. These data suggested that LjPL-3 and LjPL-2 participate in immune response through direct bacterial killing and MO/MФ activation.
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| http://dx.doi.org/10.1016/j.dci.2023.104726 | DOI Listing |
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