AI Article Synopsis
- During the drug discovery process, cell viability assays are crucial for assessing cell health and response to drugs in laboratory settings.
- Optimizing these assays ensures consistent and reliable results, utilizing metrics like IC50 and AUC to identify potential drug candidates for further testing.
- The text details a protocol using the resazurin reduction assay on MCF7 breast cancer cells, highlighting its efficiency and ease of use for drug sensitivity screening.
Article Abstract
During the preclinical stages of the drug discovery process, cell viability assays are fundamental tools for studying the phenotypic properties and overall health of cells following in vitro drug sensitivity screens. Therefore, it is important to optimize your viability assay of choice to obtain reproducible and replicable results, as well as use relevant drug response metrics (e.g., IC50, AUC, GR50, and GRmax) to identify candidate drugs for further evaluation in vivo. Herein, we used the resazurin reduction assay which is a quick, cost-effective, simple-to-use, and sensitive method for examining the phenotypic properties of cells. Using the MCF7 breast cancer cell line, we provide a detailed step-by-step protocol for optimizing drug sensitivity screens using the resazurin assay.
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| http://dx.doi.org/10.1007/978-1-0716-3052-5_18 | DOI Listing |
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