Over the last years, there is accumulating evidence that acidic organelles can accumulate and release Ca upon cell activation. Hence, reliable recording of Ca dynamics in these compartments is essential for understanding the physiopathological aspects of acidic organelles. Genetically encoded Ca indicators (GECIs) are valuable tools to monitor Ca in specific locations, although their use in acidic compartments is challenging due to the pH sensitivity of most available fluorescent GECIs. By contrast, bioluminescent GECIs have a combination of features (marginal pH sensitivity, low background, no phototoxicity, no photobleaching, high dynamic range and tunable affinity) that render them advantageous to achieve an enhanced signal-to-noise ratio in acidic compartments. This article reviews the use of bioluminescent aequorin-based GECIs targeted to acidic compartments. A need for more measurements in highly acidic compartments is identified.
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| http://dx.doi.org/10.1016/j.bbamcr.2023.119481 | DOI Listing |
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