AI Article Synopsis
- The stability of DNA origami structures in water is influenced by cations that help reduce electrostatic repulsion between the DNA strands.
- The study investigates how different concentrations of magnesium ions affect the melting temperatures of these nanostructures, revealing significant differences from predicted values, especially at high ionic strength.
- These deviations suggest that the overall stability of DNA origami at high ionic strength is largely driven by mechanical strain rather than just electrostatic interactions.
Article Abstract
The stability of DNA origami nanostructures in aqueous media is closely tied to the presence of cations that screen electrostatic inter-helix repulsion. Here, the thermal melting behavior of different DNA origami nanostructures is investigated in dependence on Mg concentration and compared to calculated ensemble melting temperatures of the staple strands used in DNA origami folding. Strong deviations of the measured DNA origami melting temperatures from the calculated ones are observed, in particular at high ionic strength where the melting temperature saturates and becomes independent of ionic strength. The degree of deviation between the measured and calculated melting temperatures further depends on the superstructure and in particular the mechanical properties of the DNA origami nanostructures. This indicates that thermal stability of a given DNA origami design at high ionic strength is governed predominantly not by electrostatic inter-helix repulsion but mostly by mechanical strain.
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| http://dx.doi.org/10.1002/cbic.202300338 | DOI Listing |
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